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It outlines extraction and purification methods, advanced spectroscopic and chromatographic characterization techniques, and underlying antioxidant mechanisms such as radical scavenging, metal chelation, and signalling modulation

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Brown adipose tissue (BAT), brain (cortex), heart, intestine, kidney, liver, lung, muscle, spleen and white adipose tissue (WAT) were dissected and quickly frozen in liquid nitrogen immediately and stored at −80 °C until metabolite extraction